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Messages - treysolid

Pages: [1] 2 3 ... 140
1
The New Joe Montgomery Birther Pit / Re: Possible WW3 thread
« on: February 27, 2022, 01:12:29 PM »
Is there a path to Russia being bros w/ the west now? They have a lot of valuable crap. It would be nice if they were bros and we could all trade and party together.
Sadly our government is full of people still fighting the Cold War.  The same people who share our intelligence with China.

Only insane people want to see a military coup in Russia.

Putin is on record as saying the fall of the USSR is the worst thing that ever happened to Russia but we're the ones still fighting the Cold War?

2
Great work here (not from the booing crowd obviously)  :thumbs:

https://twitter.com/NoSpinNews/status/1472934353583984641

https://twitter.com/NoSpinNews/status/1472983120601403400

I particularly liked this line.
I despise him less after watching that.

Still lying like crazy. There is no way the vaccine was going to take 5-12 years under normal circumstances.

3
IMHO, I think the way to get these stupid eff non chippers to get chipped is too let the card carrying chipped to go without masks.  It makes total sense, since once you're chipped you can't get it (not true) and you can't spread it (also not true).  Most of them are cry babies and hate masks.  They will quickly and angary get chipped out of jealously of the privileged chipped.

4
You can absolutely lay some of the issues at the feet of the we’re all going to die crowd.

They were right tho. Could you imagine this country going through what India is? Because it absolutely would look like that without the same precautions people are now crying about. Don't you continue to thank trump and operation warp speed? No way that happens as quickly as it did without the cautious pushing the conversation.
Anthony Fauci historically has been almost criminally cautious when it comes to the development of new treatments and vaccines.  The AIDS movement essentially wanted to assassinate the guy. 

So yes, everyone should be thanking Trump. If it were up to normal Fauci, we probably still wouldn’t have a vaccine.

you still don't understand what the NIH does and does not do, huh?

5
the dog was never truly free bc he was always on a chain

6
The New Joe Montgomery Birther Pit / Re: the post-trump gop
« on: March 27, 2021, 01:32:26 AM »
listen guys, i don't "do" cocaine - i just like how it smells

7
Other Sports (Tiger's Back) / Re: KSU Booze Cats
« on: March 20, 2021, 04:03:12 PM »
Carson Seymour going today. Personally, I think he's a bit overrated. His stuff is fine, but he has real control issues.

8
Am currently in the process of moving and came across my bball card collection. I pretty much stopped collecting in '96 but I managed to find a Kobe rookie card before I hung 'em up. Gonna sock that one away for a while.

9
Other Sports (Tiger's Back) / Re: The Cardinals
« on: March 11, 2021, 01:06:52 AM »
Let the Devil Magic commence!  :love: :love:

10
I'm confused by the emphasis that it was an outdoor mall yet he makes multiple references to having to "go into a store"  :dubious:

It was an outlet mall

11
"I don't have anger issues"

12
The New Joe Montgomery Birther Pit / Re: Trump's Taxes
« on: February 25, 2021, 03:10:26 PM »
He set up a grifting foundation WAAAAY before he got into politics

13
Thank Jesus for those coal pipelines, pumping coal all over this great nation. God bless!

14
The New Joe Montgomery Birther Pit / Re: The Biden (interim) Dictator
« on: January 27, 2021, 10:46:03 AM »
Working to dismantle a system that incentivizes people losing their freedom and allows other people to profit off of that? Sure, I'll support that - no matter who does it, when they do it, or how they previously felt about it at any point in time.

15
The New Joe Montgomery Birther Pit / Re: The Biden (interim) Dictator
« on: January 27, 2021, 12:06:49 AM »
All for ending private prisons. It's evil and should be illegal. Do the feds have to honor any existing contracts or can they stop right away?

16
The New Joe Montgomery Birther Pit / Re: Dax verbiage
« on: January 24, 2021, 07:39:09 AM »
blueanon = people who acknowledge that republicans authentically and fervently believe in Qanon

17
Congrats #blueanon nation.

You're going to start seeing a reduction in positive cases, because almost right on cue the WHO has issued guidance on Ct.   Soon to come to the U.S. if not already here.   

Instead of Ct values of 37-40 in PCR testing, we'll see Ct values of 30 . . . tops.   Thus reducing the amplification of already low viral loads that in the vast majority of cases are both asymptomatic and non-infectious. 

Cases are most certainly going to drop simply by this measure alone.   How appropriately timed . . . should have bet on it.

There's only one state that requires Ct value reporting, Florida.

As someone who designs and does qPCR assays all the time - anything that comes up after cycle 30-32 is pretty much trash (spurious products, primer dimers, etc.). Reducing your cycles by 10 will save you ~15 mins per run and allow you to process more samples a day. Good, common sense molecular biology - especially for any diagnostics lab.

Yes. So the US using a 40-cycle PCR for the last 10 months or so seems like a poor choice then.

Another science nerd here so I'll also chime in. Look at nearly any qPCR experiment and you'll see you always run them for at least 40 cycles. It's where the signal crosses a set threshold that gives you a Ct value. And that part is a bit fuzzy too since depending where you set your threshold point, you can swing that Ct value at least 3-5 cycles.

Non-nerd here, but find this stuff fascinating. Cycles are exponential as well, correct? Like, if you get a test and your showing covid after 10 cycles you’ve probably got a good but in you. But if it’s 30-35... 40, you may have like a couple of little viral guys maybe hanging out in there?

I’d love to gain a nutshell/layman’s understanding of how this all works.

Hi nicname - sorry, I just saw this. This wikipedia page is a good place to start: https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction

Because you double the number of template strands every cycle of PCR, it is an exponential process - at least, in the beginning (the curve stops being exponential at a certain point as you start running out of reagents in the reaction). That's why the threshold to determine Ct is set low, you want to measure the reaction while the amplification is still exponential. And since everything is exponential, 10-fold differences in starting template concentration will be separated by ~3.32 Ct. So if you've got 100 million viral guys crossing the threshold at cycle 7, then samples with 100 viral guys will cross the threshold at ~ cycle 27. (And 10 viral guys will be detected at ~ cycle 30).

18
Sometimes, when my eyes are quickly scanning the thread page, I read this thread as "Another Round of Applesauce For Kansas"

19
As the more contagious strain continues to spread and overtakes the progenitor strain, I don't think "feel better" will be the reality for most people.

20
I don't think this change will affect the ability to detect positive samples very much, if at all.

21
Congrats #blueanon nation.

You're going to start seeing a reduction in positive cases, because almost right on cue the WHO has issued guidance on Ct.   Soon to come to the U.S. if not already here.   

Instead of Ct values of 37-40 in PCR testing, we'll see Ct values of 30 . . . tops.   Thus reducing the amplification of already low viral loads that in the vast majority of cases are both asymptomatic and non-infectious. 

Cases are most certainly going to drop simply by this measure alone.   How appropriately timed . . . should have bet on it.

There's only one state that requires Ct value reporting, Florida.

As someone who designs and does qPCR assays all the time - anything that comes up after cycle 30-32 is pretty much trash (spurious products, primer dimers, etc.). Reducing your cycles by 10 will save you ~15 mins per run and allow you to process more samples a day. Good, common sense molecular biology - especially for any diagnostics lab.

22
The New Joe Montgomery Birther Pit / Re: The Trump Presidency
« on: January 20, 2021, 10:34:30 AM »
eff you, Donald Trump.

23
Also, the coral bedrock that FL sits on is crazy hard. It would chew up those boring drills so quick.

24
I feel like we've heard from this guy before

Quote
Copied and pasted....
Letter to Pelosi
Wow! This man is really torqued!!!  Could we wonder what Pelosi did to him to make him so mad?
FYI, This has been checked via Snopes, who contacted Mr. Guthrie, who confirmed he did write & send this letter to Ms. Pelosi,,,, here is one brave American, AND his credentials are impressive! Check his credentials, then read his letter to Pelosi!
___________________________________________________
Born: St. Louis, Missouri. August 21, 1944
Bar Admissions: North Carolina, 1969
U.S. District Court, Eastern, Middle and Western Districts of North Carolina, 1969
U.S. Tax Court
Fourth Circuit Court of Appeals
Education: Woodford College, 1966 A.B.
Mercer University, 1969 J.D.
Phi Alpha Delta
Vice-Justice, District XIV, 1968 - 1969
Professional Associations and Memberships:
North Carolina and American Bar Associations ( Member, Sections on: Administrative Law; General Practice; Litigation)
26th Judicial District and North Carolina State Bar
Mecklenburg County Bar Association
American Association of Justice
North Carolina Trial Lawyers Association
Captain, U.S. Army, 1969-1971, Vietnam
National Defense Medal, 1969
Republic of Viet Nam Service Media.
Bronze Star Medals (2), 1971
Assistant District Attorney, Mecklenburg County, 1971 - 1974
Charlotte Chamber of Commerce
Chairman, Board of Trustees - Providence United Methodist Church
Board of Directors, Alexander Children's Home
Wofford Alumni Executive Council
Scoutmaster, Boy Scouts of America
Board of Directors, Boy Scouts of America of Mecklenburg County
Board of Directors, Girl Scout Council
Life Member, National Eagle Scout Association
Life Member, Girl Scouts of America
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dear Ms. Pelosi:
I write to you out of utter disdain! You are as despicable and un-American as the traitor Jane Fonda.
I am a soon to be a 75 year-old who has voted in every state and local election since 1966.  I have voted for both Republicans and Democrats alike.  I have worked on campaigns for both Republicans and Democrats, white and black.  I served the country that I love in Vietnam, as my son did in the Middle East .  I was awarded two bronze stars.  I have been involved in politics since age 6 when my father was campaign manager for a truly great American Congressman, Charles Raper Jonas, who worked for his constituents and his country, and was to be admired, unlike you.
You obviously haven't read the Constitution recently, if ever, the Federalist Papers, or even David McCulloughs book on John Adams. You ought to take the time while riding around in your government provided luxury executive jet to do just that. You represent Socialistic and even Marxist principals that our founding fathers tried to avoid when setting out the capitalistic republican form of government represented by our Constitution.
I find it interesting that you and your husband are multi-millionaires with much of your fortune being made as a result of your public service. You have controlled legislation that has enhanced your husband's investments both on and off shore.  At the same time you redistributed the wealth of others.  Our system of a free market economy is being destroyed by the likes of you.  You ride around in a Gulfstream airplane at the tax payers expense while criticizing the presidents of companies who produced something for the economy.  You add nothing to the economy of the United States; you only subtract therefrom.
I would like to suggest that you return to the city of fruitcakes and nuts and eat your husband's canned tuna and pineapple - produced by illegal immigrants and by workers who have been excluded from the protection that 90% of the legal workers in the United States have.
I await your defeat in the next election with glee...
Don’t ever use the term un-American again for protesters who love this country and are exercising their rights upon which this country was founded.  By the way, while I served in the Army, I was spit on by the same type of lunatics who support you and who you probably supported in the 60s and 70s.  You are an embarrassment to all of us who served so that you would have the protected right of free speech to call us un-American.  But at the same time, I have the right to write you to notify you that I consider you to be un-American, as do the majority of the people of this formerly great country.  You are a true disgrace to most of the people who served this country by offering themselves for public service in the United States Congress.
I feel certain your aides will not share this letter with you, but I intend to share it with many...
cid:169d1919c5b7e28bb431
cid:169d1919c5b7e36d2c42
Let's help this man fulfill his 'commitment' to Pelosi by sharing his remarks.

Straight from graduating law school and passing the bar to being a **CAPTAIN** in the army. rough ridin' overachiever.

25
The New Joe Montgomery Birther Pit / Re: LOL TRUMP
« on: January 14, 2021, 01:53:00 PM »
Something I thought of last night, was Jan 6th his last rally? Will any venue take the chance that he'll instruct his cult to do more violence?

Didn't he just have a rally in Alamo, TX?

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